Table 3.
Comparison statistics measured for PCR and LAMP amplification for Schistosoma mansoni based on two different extraction techniques
| PCR-Qiagen (95% CI) | PCR_LAMP-PURE (95% CI) | LAMP-Qiagen (95% CI) | LAMP_LAMP_PURE (95% CI) | |
|---|---|---|---|---|
| Sensitivity | 83% (74–89%) | 79% (69–86%) | 100% (96–100%) | 88% (80–93%) |
| Specificity | 100% (59–100%) | 31% (9–61%) | 100% (59–100%) | 14% (0.4–58%) |
| Prevalence | 94% | 88% | 94% | 94% |
| PPV | 100% | 90% | 100% | 94% |
| NPV | 28% | 16% | 100% | 7% |
| Accuracy | 84% | 73% | 100% | 83% |
LAMP-PURE = loop-mediated isothermal amplification-Procedure for Ultra Rapid Extraction; NPV = negative predictive value; PCR = polymerase chain reaction; PPV = positive predictive value. The infection prevalence identified by each extraction technique is highlighted in bold to emphasize the difference.