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. 2019 May 13;101(1):101–107. doi: 10.4269/ajtmh.19-0097

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Figure 1. — (A) Sensitivity of LAMP assays for the detection of Leishmania DNA using serial dilutions of Leishmania tropica. After incubation at 67°C for 50 minutes, LAMP reactions were inspected by agarose gel electrophoresis of LAMP products. Lanes 1–6 show the typical ladder-shaped pattern of a positive reaction. (B) Specificity of Leishmania major LAMP assay tested on different species: lane 1: 1-kb ladder, lane 2: L. major (L3171), lane 3: Leishmania infantum (MHOM/TN/1980/IPT1), lane 4: L. tropica (AM), lane 5: Leishmania donovani (PKDL306), and lane 6: negative control.