Table 3.

Performance of the different assays for the diagnosis of CL by classical validation (2 × 2) and through LCA (95% CI)

	Sensitivity (%)		Specificity (%)
	2 × 2 analysis	LCA	2 × 2 analysis	LCA
qPCR	100*	100 (95.2–99.9)	100*	73.3 (65.3–81.3)
Microscopy	82 (70–90)	88.1 (83.1–93.1)	90.9 (70–98)	80 (68.9–84.4)
LAMP	84 (71.1–91.8)	100 (98–100)	100 (82–100)	100 (96–100)
PCR	54 (40.4–67)	64.3 (57.0–71.6)	100 (82.1–100)	100 (89–100)

LAMP = loop-mediated isothermal amplification; LCA = latent class analysis; qPCR = quantitative PCR.

* By definition (qPCR reference test).