Table 4.
Details and summary of the diagnostic performance of different studies using LAMP for CL diagnosis compared with our study
| Kothalawala et al.50 | Adams et al.51 | Verma et al.49 | Verma et al.52 | This study: 3 different species-specific LAMP | |
|---|---|---|---|---|---|
| Country | Suriname | Colombia | India | India | Tunisia |
| LAMP target | 18S rRNA | 18S rRNA | kDNA | kDNA | cpb |
| Primer specificity | L. donovani | Leishmania genus | L. donovani, L. major, L. tropica | L. donovani | L. major, L. tropica, L. infantum |
| Study population | 17 CL | 105 CL | 10 CL | 62 PKDL | 82 CL suspects |
| Clinical specimen tested | Lesion aspirate | Lesion swab | Skin biopsy | Skin biopsy | Lesion aspirate |
| DNA purification method | Boom method | Qiagen DNAeasy blood and tissue kit | QIAamp Mini Kit (QIAGEN, Hilden, Germany) | QIAamp Mini Kit (QIAGEN) | Wizard® Genomic DNA Purification kit (Promega) |
| Reference test | Microscopy | Microscopy and/or culture | rK39 RDT-positive qPCR | rK39 RDT-positive qPCR | qPCR |
| Sensitivity (%) | 82.6 | 95 | 80 | 97 | 84 |
| Specificity (%) | 100 | 86 | 100 | 100 | 100 |
CL = cutaneous leishmaniasis; cpb = cysteine protease B; L. = Leishmania; LAMP = loop-mediated isothermal amplification; qPCR = quantitative PCR.