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. 2019 May 6;101(1):198–206. doi: 10.4269/ajtmh.18-0658

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 3. — (A) Western blot of purified scFvs (3-2, 4-2, 10-3, 13-1, 16-1, 18-1 22-1, 27-5, 35-2, and 39-1). ELISA results for purified novel anti-EBOV scFv4-2 (B) and scFv22-1 (C) (generated by ribosome display), commercial monoclonal (mouse anti-EBOV antibody 6D8 and mouse anti-SUDV ebola virus antibody), and polyclonal (rabbit anti-RESTV GP, rabbit anti-BDBV GP, rabbit anti-MARV GP, and rabbit anti-TAFV GP) antibodies as positive controls, and an scFv negative control (mouse anti-PilB scFv21 antibody). BDBV = Bundibugyo virus; EBOV = Ebola virus; GP = glycoprotein; MARV = Marburg virus; SUDV = Sudan virus; TAFV = Tai Forest virus. This figure appears in color at www.ajtmh.org.