Fig. 2. CD19 CAR T cells expressing tPSMA maintain function in vitro and in vivo.
(A) Mock or CD19-tPSMA CAR T cells were incubated with K562-CD19-NLR target cells at a ratio of 4:1. Target cells were analyzed every 2 hours using an IncuCyte live cell imaging system. (B) A kill index [1/area under the curve (AUC)] was calculated at the indicated effector:target ratio. (C and D) Nalm6-GFP-fLuc tumor cells were injected intravenously into female NOD scid (nonobese diabetic severe combined immunodeficient) gamma (NSG) mice (n = 8 per group) on day 0, and 1 × 106 mock or CAR T cells were injected on day 4. Whole-body bioluminescence imaging (BLI) was performed to determine tumor burden, with data displayed as average radiance for each group (C), and survival events were recorded (D).