TABLE 2.
Oligonucleotide primers used in this study
| Primer purpose and name | Sequence (5′–3′) |
|---|---|
| mrdA (PBP2) sequencinga | |
| SR176 | CATCACCACCAACCATCCTT |
| SR177 | CCGTGCAGCACATCTTCATA |
| SR178 | TGACGATATTGCTGCATTCC |
| SR179 | GGTTCACCAGCGGTGTATTC |
| SR180 | TGGTTTCCACGCCTAGTTATG |
| SR181 | AGGTTTCGTTCGCTTTCAGA |
| SR182 | CCGAATGGATGGGTAAATTC |
| SR183 | TGTGGGATCGAGATGGACTT |
| Gene manipulations and diagnostic PCR | |
| SR200 | TTGACGGTATCTCCAGCAAA |
| SR201 | GCTAAGGCCAGAGAGGAACA |
| SR202 | ACACCATTCCGGTTGGTATC |
| SR203 | TACGCTCCATCATGCCAATA |
a
The mrdA gene was amplified from clinical isolates for sequencing in segments using primer pairs SR176/SR177, SR178/SR179, SR180/SR181, SR182/SR183, and SR176/SR183.