TABLE 2.
PCR primers used in this study
| Gene target | Forward primer(s) (5′–3′) |
Reverse primer(s) (5′–3′) |
Component(s) surveyed |
Sample site(s) |
Reference(s) |
|---|---|---|---|---|---|
| Bacterial 16S rRNA gene V6 region |
CTAACCGANGAACCTYACC, CNACGCGAAGAACCTTANC, CAACGCGMARAACCTTACC, ATACGCGARGAACCTTACC |
CGACRRCCATGCANCACCT | All | UWM RAS | 72 |
| Bacterial 16S rRNA gene V4-V5 region |
CCAGCAGCYGCGGTAAN | CCGTCAATTCNTTTRAGT, CCGTCAATTTCTTTGAGT, CCGTCTATTCCTTTGANT |
All | All | 73 |
| Archaeal 16S rRNA gene V4-V5 region |
GCCTAAAGCATCCGTAGC, GCCTAAARCGTYCGTAGC, GTCTAAAGGGTCYGTAGC, GCTTAAAGNGTYCGTAGC, GTCTAAARCGYYCGTAGC |
CCGGCGTTGANTCCAATT | Biofilters | All biofilter samples | 74 |
| Betaproteobacterial amoA | GGGGHTTYTACTGGTGGT | CCCCTCKGSAAAGCCTTCTTC | Biofilters | All Biofilter Samples | 75, 76 |
| Comammox amoA | GGAYTTYTGGNTNGATTGGA | WRKTNNGACCACCASKACCA | Biofilters | All biofilter samples | Modified from reference 58 |
| Nitrospira nxrB | TACATGTGGTGGAACA | CGGTTCTGGTCRATCA | Biofilters | All biofilter samples | 53 |