FIG 2.

Assay validation. (A) Determination of robustness across CHIKV genotypes. New assays (boldface) and reference assays were tested with CHIKV RNA of the Asian, ECSA, and West African genotypes. (B) Determination of lower limits of detection (LOD) for the new PCR assays. Quantified RNA of the Asian CHIKV genotype was diluted stepwise and was tested in eight replicates with first-round assays. Probit analyses were done using R 3.5.2. (C) Conservation of primer target regions among representative Mayaro (NC003417) and O'nyong-nyong (NC001512) viruses. Mismatches with the new CHIKV assays are highlighted.