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. 2019 Jun 25;10(41):4125–4138. doi: 10.18632/oncotarget.27010

Figure 3. The pattern of expression of miRNA processing intermediates suggest a defect in the transcription of miR-15/16 primary transcripts.

Figure 3

RT-qPCR was used to test the endogenous expression of miR-15/16 processing intermediates including host gene mRNA/lncRNA and pri-miRNA expression in the MPM cell line panel. (A) pri-miR-15a and pri-miR-15b also show downregulated expression in all MPM cell lines compared to MeT-5A (red dotted line). (B) host genes DLEU2 and SMC4 are transcribed with miR-15a/16-1 and miR-15b/-16-2 respectively and show downregulated expression in MPM cell lines compared to MeT-5A. Expression values were normalized to 18S expression, and are relative to levels in MeT-5A. Results are the mean of 3 biological replicates ± SEM. ** = p ≤ 0.01.