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. 2018 Jan 2;10(41):4192–4204. doi: 10.18632/oncotarget.24115

Figure 5. ELK1 and STRN4 are direct targets of miR-873.

Figure 5

(A, B) qRT-PCR analysis of mRNA levels of candidate genes in LoVo-control and LoVo-miR-873 cells (A) and HCT116-control and HCT116-miR-873 cells (B). (C) Dual Luciferase reporter assays of 3ʹUTRs of candidate genes upon transfection of miR-873 in 293T cells. (D) Schematic of mutations generetated in miR-873 binding regions in 3ʹUTRs of ELK1 and STRN4. (E) Dual Luciferase reporter assays of wild-type or mutant 3ʹUTRs of candidate genes upon transfection of miR-873 in 293T cells. (F) qRT-PCR analysis of ELK1 and STRN4 mRNA levels in HCT8-NC inhibitor and HCT8-miR-873 inhibitor cells. (G) Western blotting analysis of ELK1 and STRN4 protein levels in LoVo/HCT116-control, LoVo/HCT116-miR-873 cells, HCT8-NC inhibitor and HCT8-miR-873 inhibitor cells. *P < 0.05; **P < 0.01; ***P < 0.001; N.S. no significance.