Skip to main content
. 2019 Jun 25;10(41):4091–4106. doi: 10.18632/oncotarget.26983

Figure 4. Proapoptotic effect of Cannabis extracts on cancer cells.

Figure 4

A549 cells were incubated for 24 hours with 4 or 8 μg/mL CAN5, CAN9, CAN10 extracts or with DMSO (control). Apoptosis (early and late) was assessed by Annexin V/PI staining using flow cytometry. Results were calculated as % of positive annexin V-FITC cells out of total cells counted. (A) Representative dot plots of cells treated with 4 or 8 μg/ml CAN5, CAN9 and CAN10 extracts. (B) Bar chart of total apoptosis following incubation with extracts. Data are presented as mean ± SE (N=5). Asterisks indicate statistically significant differences compared to control (***P < 0.0001; two-way ANOVA with Bonferroni's post hoc multiple comparisons test). (C) Cells were lysed and resolved on 15% SDS-PAGE followed by western blotting with anti-cleaved caspase 3 and Anti β-Tubulin antibodies.