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. 2019 Jun 25;10(41):4091–4106. doi: 10.18632/oncotarget.26983

Figure 6. Selectivity of Cannabis extracts on the survival of human cells.

Figure 6

(A) A549 and NCI-H460 lung carcinoma cells and normal bronchial epithelial cells (Normal AECs) were incubated with 4 µg/ml of CAN1-12 or control (ctl). Data are reported as mean ± SE of percentage of dead cells out of total cells relative to control treatment (N=3). Asterisks indicate statistical differences between cancer vs. normal AECs treated with Cannabis extracts (*P < 0.05, **P < 0.005, ***P < 0.0005; two-way ANOVA with Bonferroni's post hoc multiple comparisons test). (B, D) Representative brightfield images of normal AECs and lung carcinoma (NCI-H460, A549) (B) or HT-29 colon carcinoma (D) cell lines treated with control or CAN5. (C) Dose-response curve of HT-29 cells after 24 hours incubation with or without (control) 2-10 μg/ml of CAN1-CAN12. Data are presented as mean ± SE (N=9). (E) Screening results of 4 μg/ml of 43 different Cannabis extracts, or control on the survival of HT-29 cells (N=1). (F) Cannabimimetic receptors CNR1 (CB1), CNR2 (CB2), GPR55, TRPV1, TRPA1, TRPV2, TRPM8 mRNA levels were evaluated by qPCR. Expression levels were represented as ΔCT levels of the receptors. *Jurkat cell line was added as a control for CNR2 expression. Results are presented as mean expression of N=3 and normalized to the GUSB housekeeping gene. Values were color-coded according to the magnitude of expression. UD - Under Detectable Level. The higher the ΔCT values are the lower receptor expression.