Metastatic tumor cells in bone have been shown to secrete a range of proteins including IL-6, IL-11, and PTHrP [26, 27]. This results in increased expression of RANKL
on osteoblasts, RANKL can then bind RANK receptors on osteoclasts and osteoclast precursor cells resulting in activation of AP-1
and NF-κB transcription factors [26, 28].
AP-1 and NF-κB activation results in osteoclast differentiation, maturation, fusion, and ultimately increased bone
resorption, which releases previously sequestered growth factors such as TGF-β and IGF-1 that stimulate tumor cell
proliferation, in turn increasing PTHrP expression, resulting in reinforcement of the ‘vicious cycle’ of bone
metastases [23, 26, 28].
AP-1, activator protein 1; IGF-1, insulin-like growth factor 1; IL, interleukin; PTHrP, parathyroid hormone-related
protein; RANK, receptor activator of NF-κB; RANKL, receptor activator of NF-κB ligand; TGF-β, transforming
growth factor β.