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. 2019 Jun 27;10:828. doi: 10.3389/fpls.2019.00828

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Copyright © 2019 Chen, Peng, Gao, Zhang, Liu, Fu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in NCED content and activity in imbibed seeds in the absence or presence of coumarin. Seeds were imbibed at 28°C in water, or in 0.5, 1, or 2 mM coumarin. Seed samples were collected at different times and stored at −80°C for NCED content (A) and NCED activity (B) determination. NCED content and activity were determined ELISA. Data represent the mean ± SE of three biological replicates each of 1 g of embryos. Means denoted by the same letter did not significantly differ at p < 0.05 according to Fisher’s least significant difference test.