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. 2019 Jun 27;10:828. doi: 10.3389/fpls.2019.00828

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Copyright © 2019 Chen, Peng, Gao, Zhang, Liu, Fu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical staining to assess seed viability, O₂⁻ and H₂O₂ content, and peroxidase activity during germination of rice seeds in water or coumarin. Seeds were imbibed in water or 1 mM coumarin for 6, 18, or 36 h, after which whole seeds and slit half seeds were stained. (A) Seed viability was assessed by staining with TTC. (B) O₂⁻ content was assessed by staining with NBT. (C) H₂O₂ content was assessed by staining with DAB. (D) Peroxidase activity was assessed by staining with TMB.