Figure 1.

Immunoperoxidase staining (A–H) and double indirect immunofluorescence (I–I”) methods to analyse Ten-2 immunolabeling pattern in histological section of adult rat cerebral cortex. (A–C), control group, observe neurons in cerebral cortex layer V exhibiting discrete immunoreactivity to Ten-2, homogenously distributed in cell cytosol (large arrow) or associated with cell membrane (small arrows). In (D–H), mechanical brain lesion in cerebral cortex from 48 h postoperative period group, showing strong immunoreactivity to Ten-2 in reactive astrocytes. In (E,F), note Ten-2-LI reactive astrocytes with palisading aspect sending some cell extensions (arrows) to haemorraghic area (F, black asterisk). Ten-2-LI hipertrophic reactive astrocytes are densely grouped immediately below (G) and decreasing toward deeper layers of the cerebral cortex (H). Reactive astrocytes exhibited large cell bodies with numerous long arborized cell extensions (G,H), sometimes encircling the blood vessel (H, arrow). In (I–I”) immunolabeling pattern of Ten-2 in reactive astrocytes (GFAP-LI) analyzed by confocal microscopy. Observe that immunolabeling to Ten-2 is distributed in the cytosol of the cell body and cell extensions (arrows), exhibiting a punctiform pattern and sometimes associated with plasmatic membrane. GFAP-LI, glial fibrillary acidic protein-like immunoreactive; Ten-2-LI, teneurin-2-like immunoreactive; V, cerebral cortex layer V.