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. 2019 Jun 27;13:655. doi: 10.3389/fnins.2019.00655

Figure 2.

Figure 2

Temporal analysis of Ten-2-LI reactive astrocytes. (A–E”) confocal microscopy photomicrographs showing double immunolabeling in reactive astrocytes (GFAP-LI/ Ten-2-LI) with nuclear DAPI staining in cerebral cortex from control, 24 h, 48 h, 3 and 5-day groups. Note that these reactive astrocytes are clearly visible at 24 h (B–B”) and nearly all of them exhibit immunoreactivity to Ten-2 in all experimental groups. (F) quantitative analysis of absolute values (mean ± SEM) of astrocyte cell profiles exhibiting immunoreactivity to Ten-2 (GFAP-LI/Ten-2-LI) in all experimental groups (n = 5, five animals per group). Note that reactive astrocyte cell profiles (GFAP-LI) showed no statistical difference among all groups, indicating that there is no cell proliferation. However, note that reactive astrocyte cell profiles (GFAP-LI/Ten-2-LI) significantly increased (p < 0.0001) in all experimental groups with mechanical brain injury compared to control group. Mean (± SEM) values from each group were submitted to one-way ANOVA and Dunnett's multiple comparisons post hoc test, considering p < 0.05 as significant. * Statistically significant difference when compared with control group (p < 0.0001). GFAP-LI, glial fibrillary acidic protein-like immunoreactive; Ten-2-LI, teneurin-2-like immunoreactive.