Figure 3.

LPHN-LI reactive astrocytes. Confocal microscopy photomicrographs showing reactive astrocytes (GFAP-LI) stained with nuclear DAPI and exhibiting immunoreactivity to LPHN-1 (A–A”), LPHN-2 (B–B”), and LPHN-3 (C–C”) in cerebral cortex with mechanical brain lesion in cerebral cortex from 3-day postoperative period group. Note that nearly all reactive astrocytes exhibited substantial immunoreactivity to LPHN-1 (A-A”), moderate to LPHN-3 (C–C”) and modest to LPHN-2 (B–B”) (arrows). Ab1 (LPHN-1), omission of LPHN-1 primary polyclonal antibody; GFAP-LI, glial fibrillary acidic protein-like immunoreactive; LPHN-1-LI, latrophilin-1-like immunoreactive; LPHN-2-LI, latrophilin-2-like immunoreactive; LPHN-3-LI, latrophilin-3-like immunoreactive; ^*, cortical lesion area.