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. 2019 Jun 27;13:655. doi: 10.3389/fnins.2019.00655

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Copyright © 2019 Tessarin, Michalec, Torres-da-Silva, Da Silva, Cruz-Rizzolo, Gonçalves, Gasparini, Horta-Júnior, Ervolino, Bittencourt, Lovejoy and Casatti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ten-2/TCAP-2 genic expression. (A) conventional RT-PCR products using total RNA extracted from cerebral cortex of all experimental groups (n = 4, four animals per experimental group) and Ten-2, TCAP-2 and neurofilament light (NFL) primers, visualized in 1.5% agarose gel stained with bromide ethidium. (B) Semi-quantitative analysis of relative gene expression (mean ± SEM) of Ten-2 and TCAP-2 bands normalized in relation to GADPH and expressed in relation to NFL. Mean (± SEM) values from each group were analyzed by one-way ANOVA and Dunnett's multiple comparisons post hoc test, considering p < 0.05 as significant. TCAP-2: + statistically significant difference when compared with control group (p < 0.0001); ++ statistically significant difference when compared with 24 h group (p < 0.0001). Ten-2: ^*statistically significant difference when compared with 24 h group (p < 0.05) ^**statistically significant difference when compared with control group (p < 0.001); ^***statistically significant difference when compared with control group (p < 0.0001); # statistically significant difference when compared with 24 h group (p < 0.0001). R1-R20, animal number identification.