Table 1.
Primer sets used in RT-PCR and PCR assays.
| Gene | Primer Pair | Primer sequence (5′ to 3′) | Exon | Product size (base pairs) |
|---|---|---|---|---|
| In vivo RT-PCR ANALYSIS | ||||
| Teneurin 2 | mTen2e23FWD | 5′-gatgtcaactgcatctgctactc-3′ | 23 | 491 |
| NM_011856.4 | mTen2e23RVS | 5′-agtccagtgttcccatcataagtc-3′ | ||
| TCAP-2 | mTCAP2e28FWD | 5′-gacaagatgcactacagcatcgag-3′ | 28 | 496 |
| NM_011856.4 | mTCAP2e28RVS | 5′-ccatctcattctgtcttaagaactgg-3′ | ||
| NFL | rNFLe1FWD | 5′-atcagcaacgacctcaagtctatccgc-3 | 1 | 761 |
| NM_010910.2 | rNFLe1RVS | 5′-gagtccagtgttcccatcataagt-3′ | ||
| GADPH | rGADPHe4FWD | 5′-ccatgacaactttggcattg-3 | 4-6 | 302 |
| BC023196.2 | rGADPHe6RVS | 5′-cctgcttcaccttcttg-3′ | ||
| In vitro PCR ANALYSIS | ||||
| β-actin | mBetaActine4FWD | 5′caggtcatcactattggcaacgag3′ | 4-6 | 357 |
| NM_007393.5 | mBetaActine6RVS | 5′ctcatcgtactcctgcttgctgat3′ | ||
| Teneurin 1 | mTen1e25FWD | 5′gtgtcacctgatggcaccctctat3′ | 25 | 402 |
| NM_011855.4 | mTen1e25RVS | 5′tcctgggtatgtcatcaaggccaa3′ | ||
| Teneurin 2 | mTen2e23FWD | 5′atcctgaactcgccgtcctcctta3′ | 23 | 405 |
| NM_011856.4 | mTen2e23RVS | 5′ctccaggttctgagtggacacggc3′ | ||
| Teneurin 3 | mTen3e22FWD | 5′agtggaatacccggtggggaagcac3′ | 22 | 427 |
| NM_011857.3 | mTen3e22RVS | 5′gtgagtaccgttgatgtcaaagatg3′ | ||
| Teneurin 4 | mTen4e22FWD | 5′atcgaccaattcctgctgagcaag3′ | 22 | 369 |
| NM_011858.4 | mTen4e22RVS | 5′catgttctgagtgttcaggaaagg3′ | ||
| TCAP-1 | mTCAP1e32FWD | 5′acgtcagtgttgaatgggaggacta3′ | 32 | 351 |
| NM_011855.4 | mTCAP1e32RVS | 5′cctcctgcctatttcactctgtctcat3′ | ||
| TCAP-2 | mTCAP2e28FWD | 5′gacaagatgcactacagcatcgag3′ | 28 | 496 |
| NM_011856.4 | mTCAP2e28RVS | 5′ccatctcattctgtcttaagaactgg3′ | ||
| TCAP-3 | mTCAP3e29FWD | 5′caacaacgccttctacctggagaac3′ | 29 | 506 |
| NM_011857.3 | mTCAP3e29RVS | 5′cgatctcactttgtcgcaagaact3′ | ||
| TCAP-4 | mTCAP4e29FWD | 5′tttgcctccagtggttccatctt3′ | 29 | 602 |
| NM_011858.4 | mTCAP4e29RVS | 5′tggatattgttggcgctgtctgac3′ | ||