Figure 15. Comparison of FBA steady-state fluxes $\nu$ to maximal fluxes $V_{\text{max}}$ obtained from protein abundances and turnover numbers from BRENDA and the literature.

Map components and labels as in Figure 5, with reaction highlighting and gene loci/gene-protein-reaction rules omitted. Each edge is colored according to the ratio between $V_{\text{max}}$ and $\nu$: Blue indicates $V_{\text{max}}>\nu$, red indicates $V_{\text{max}}<\nu$ and green indicates that no $V_{\text{max}}$ could be obtained (because of either missing turnover number or missing protein abundance; or because reaction is not enzymatic to begin with).

Figure 15—figure supplement 1. Statistics of FBA steady-state fluxes $\nu$ vs. maximal fluxes $V_{\text{max}}$ comparison (see Figure 15).

(A) Summary of $V_{\text{max}}$ vs. $\nu$ comparison over all 253 non-exchange reactions in the model. Red, blue, green: Meaning as in Figure 15. Green-striped: Subset of green set—reactions without $V_{\text{max}}$ that pertain to transport, which usually do not have an EC number associated with them. Gray: Reactions with $\nu =0$ in the FBA solution (thus $V_{\text{max}}>\nu$ always fulfilled). B: Histogram of $V_{\text{max}}/\nu$ over the blue and red subset in panel A.