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. 2019 Jul 1;8:e45919. doi: 10.7554/eLife.45919

Figure 4. PIE-Scope commander, a CLEM-friendly interface.

Figure 4.

(a) A scheme of the communication setup. Here the microscope computer controls the FIB/SEM and runs the python control server. On the support computer, we run a python client (ThermoFisher Autoscript) and the custom built PIE-scope commander to control both the FIB/SEM and the LM. (b) The PIE-scope commander interface, all the required imaging positions can be calibrated from the main window, basic imaging parameters can be set, both FIB and LM images are visible side-by-side and data location can be defined for each experiment. File naming will have a user-defined root and appendix describing the imaging parameters (e.g. FIB, SEM, LM, laser line, dwell-time, exposure time).