Fig. 3.

BML-111 inhibited the phosphorylation level of extracellular signal-regulated kinase 1/2 (ERK1/2) and signal transducer and activator of transcription 3 (STAT3). Assay the phosphorylation level of ERK1/2 and STAT3 in the placenta from the three groups. ( A – D ) The ERK1/2, p-ERK1/2, STAT3, and p-STAT3 protein expression in the placenta from the three groups were determined by Western blot analyses (β-actin served as an internal control). Densitometric analysis of the visualized bands was performed using the Gel-Pro analyzer 4 software to normalize ERK1/2, p-ERK1/2, STAT3, and p-STAT3 protein expression with the corresponding β-actin levels. Results were expressed as mean ± standard error of the mean (SEM) ( n  = 8, p  < 0.05). The data shown are representative of three independent experiments.