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. 2019 Jun 6;42(2):688–696. doi: 10.3892/or.2019.7188

Figure 2.

Figure 2.

Bmi1 promotes carcinogenicity and tumor sphere formation ability in cervical cancer. (A) The expression of Bmi1 protein in three cervical cancer cell lines was detected by western blotting; the teratoma cell line Tera-1 was used as the positive control. (B) Bmi1 protein expression was evaluated in Bmi1-overexpressing SiHa and HeLa cells by western blotting. (C) Bmi1 protein expression was evaluated in Bmi1-silenced C33A cells by western blotting. (D) Tumor growth was assessed over (E) 7 weeks, as well as the (F) the tumor net weight of nude mice inoculated with SiHa-Bmi1, SiHa-GFP, HeLa-Bmi1 and HeLa-GFP cells with three replicates (1×105 cells). Additionally, (G) tumor growth was measured over (H) 12 weeks, as well as the (I) tumor weights of nude mice inoculated with C33A-shBmi1 and C33A-shControl cells with three replicates (1×106 cells). (J) The tumor sphere formation assay was performed in FBS-free medium in low-density cultures per 200 cells with six replicates, and then (K) the number of tumor spheres formed by Bmi1-overexpressing SiHa and HeLa cells and Bmi1-silenced C33A cells was calculated. Bars=SE. *P<0.05, **P<0.01 vs. respective control. Bmi1, polycomb complex protein Bmi1; GFP, green fluorescent protein; sh, short hairpin.