Skip to main content
. 2019 Jul 3;8(1):1628592. doi: 10.1080/20013078.2019.1628592

Figure 2.

Figure 2.

Most AChE activity in serum-containing medium is not pelletable. (a) AChE activity was not detected in serum-free media formulations (AIM Vand RPMI-1640 NCM), but was found at high levels in serum-containing medium (R10 = RPMI 10% FBS NCM). Differential ultracentrifugation of AIM Vand RPMI recovered no activity in differentially centrifuged pellets (2 K, 10 K, 100 K). From serum-containing medium, only a very small fraction of total AChE activity could be recovered by differential centrifugation, and values in complete NCM. (b) After conditioning medium with 72 h culture of PM1 lymphocytic cells, similar amounts of AChE activity as in (a) could be pelleted from serum-containing medium. Comparatively smaller amounts were recovered when cells were switched to AIM V medium.