Table 1.

Screening Outcomes across Representative Targets.

Target Protein	Protein Class	Protein Mass (kDa)	Engineered/Native	Hit Rate (>3σ), %
ATG4B	Protease	44.5	Native	0.1
			Nativea	1.4
			Engineered	1.5
Lfa1	Integrin, I-domain	21.0	Engineeredb	1.8
			Engineeredb	1.6
Mac1	Integrin, I-domain	22.8	Engineeredb	2.6
			Engineeredb	2.3
LRH-1c,d	Nuclear receptor	28.3	Native	0.7
Target 1	Ubiquitin ligase	8.78	Native	1.4
Target 2	Kinase	19.3	Native	0.4
Target 3c	Kinase	37.3	Engineered	0.6
14–3-3σ	Adapter protein	26.5	Native	1.8
			Engineeredb	2.8
			Engineeredb	2.7

^aScreened in the presence of a protein partner.

^bCys-mutants targeting the same pocket on a respective target.

^cScreened 1280 of 1600 compounds.

^dde Jesus Cortez et al.²⁵