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. Author manuscript; available in PMC: 2020 Jun 4.
Published in final edited form as: Structure. 2019 Apr 4;27(6):893–906.e9. doi: 10.1016/j.str.2019.03.004

Figure 6 – Determination of the associating domains between IgLON protomers –

Figure 6 –

A – BLI curves of purified NEGR1 (100 mM) interacting with NTRI-Fc or its shorter constructs immobilized on a sensor tip, highlighting the associating deletion constructs (Ig1-Fc, Ig1–2-Fc). B – IgLON5 (195 μM) was used to test the binding of four NTRI-Fc mutants. C – The same NTRI mutants were tested by using 100 μM of purified NEGR1. D – Relative expression of the NTRI-Fc mutants in the cell culture medium. As expected, the introduction of an N-linked glycosylation site (T114N) causes a slightly slower migration of the mutant (white arrowhead).