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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: J Neurochem. 2008 Apr 1;106(1):24–36. doi: 10.1111/j.1471-4159.2008.05385.x

Fig. 4.

Fig. 4

Induction of autophagic vacuoles (AVs) by l-DOPA. a–c) GFP-LC3 indicates l-DOPA induction of AVs. Ventral midbrain neuronal cultures derived from green fluorescent protein–LC3 (GFP–LC3) transgenic mice were treated with vehicle (cnt) or 100 μM l-DOPA for 48 h. Note that the control exhibits diffuse cytosolic fluorescence, whereas puncta (arrows) are visible in the l-DOPA-treated neurons. d–f) Monodansylcadaverine label is consistent with l-DOPA induction of AVs. Wild-type ventral midbrain neuron cultures were treated with 100 μM l-DOPA (e: 24 h, f: 1 week) or vehicle labeled with 50 μM monodansylcadaverine for 1 h. AVs appear to be absent in controls and are markedly enhanced by l-DOPA. Scale bars = 10 μm.