Fig. 2.

Npm1^cA/+ depletes HSCs and expands myeloid colony-forming cells. a Schematic diagram of the design of the Npm1^frt-cA/+ allele. Asterisk indicates frameshift mutation to create a humanized mutant exon 12 (p.W288fs*12). b Frequency and c total number of LT-HSC, ST-HSC, MPP2, MPP3, MPP4, and MyPro cells in the BM of +/+ (n = 5) and cA/+ (n = 5) mice at 4 months post-tamoxifen. d Total CFU and e colony types derived from 50K BM MNCs isolated from +/+ (n = 8) and cA/+ (n = 8) mice at 4 months post-tamoxifen. Results are from four independent experiments. f Frequency of myeloid, B, and T cells within PB and g Gr-1⁺ and Gr-1⁻ cells within myeloid PB at 4 months post-tamoxifen in +/+ (n = 6) and cA/+ (n = 12) mice. In all graphs, dots represent individual mice, bars indicate mean ± s.e.m. *P < 0.05; ***P < 0.001