Fig. 6.

Myeloid-restricted progenitor cells are tumor-propagating cells in Dnmt3a^R878H/+ Npm1^cA/+ AML. a Experimental design for non-competitive tertiary transplantation of FACS-sorted Dnmt3a^R878H/+ Npm1^cA/+ BM myeloid progenitors from secondary recipient mice with AML. b Overall survival of tertiary transplant recipient mice (n = 12). Results are from three independent experiments. c WBC, RBC, and PLT counts in moribund mice (control, n = 3; +/+ cA/+, n = 9). d Spleen weights of moribund mice (control, n = 3; +/+ cA/+, n = 11). e Representative Giemsa-stained BM cytospin (far left; 40×, scale bars are 40 μm) and H&E-stained spleen (center; 10×, scale bars are 100 μm) and liver sections (far right; 10×, scale bars are 100 μm) from moribund recipient mice. In all graphs, dots represent individual mice, bars indicate mean ± s.e.m. **P < 0.01