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Representative double immunofluorescent staining of pTDP-43 and mitochondria in affected muscle fibers in IBM. The large-field and enlarged images show the colocalization between pTDP-43 and mitochondria in IBM muscle biopsy. The line-scan analysis along the solid white line depicted in the merged large-field image to the left is also shown. Green: pTDP-43; Red: mitochondria (stained by the OXPHOS antibody cocktail); Blue: DAPI.
