Extended Data Fig. 6. Local intravaginal immunization with HSV-2 is required for the recruitment of memory B cells.

C57BL/6 mice were immunized intravaginally or subcutaneously with TK⁻ HSV-2. Five weeks later, mice were challenged with WT HSV-2. a, One day after challenge, the number of memory and naïve B cells in vaginal tissues was analyzed by flow cytometry (naïve; n=4, ivag immune; n=6, SQ immune/WT HSV-2; n=5, ivag immune/WT HSV-2; n=5). b, HSV-2-specific IgG2b antibody in serum was measured by ELISA (naïve; n=3, ivag immune; n=3, SQ immune/WT HSV-2; n=5, ivag immune/WT HSV-2; n=3). Data are mean ± SEM. Data are representative of two independent experiments. Statistical significance was analyzed by two-tailed Mann-Whitney U test.