Skip to main content
. Author manuscript; available in PMC: 2019 Dec 12.
Published in final edited form as: Nature. 2019 Jun 12;571(7763):122–126. doi: 10.1038/s41586-019-1285-1

Extended Data Fig. 7. Antigen-specific recall response is necessary for the recruitment of memory B cells.

Extended Data Fig. 7

a-b, C57BL/6 mice immunized ivag with TK− HSV-2 five weeks prior were challenged with WT HSV-2 or influenza A/PR8 virus intravaginally (immune; n=6, immune/WT HSV-2; n=4, immune/PR8; n=4). a, One day after secondary challenge, the number of memory B cells in vaginal tissues was analyzed by flow cytometry. b, Indicated virus titer in vaginal wash was measured one day after secondary challenge. c, C57BL/6 mice immunized ivag with TK− HSV-2 five weeks prior were challenged with WT HSV-2 or CpG1826 intravaginally. One day after secondary challenge, the number of memory B cells in vaginal tissues was analyzed by flow cytometry (immune; n=5, immune/WT HSV-2; n=6, immune/CpG1826; n=6). d-e, C57BL/6 mice immunized ivag with TK− HSV-2 five weeks prior were challenged with active or heat-inactivated (HI) WT HSV-2 intravaginally. d, One day after secondary challenge, the number of memory B cells in vaginal tissues was analyzed by flow cytometry (immune; n=5, immune/WT HSV-2; n=7, immune/HI HSV-2; n=14). e, One day after challenge, HSV-2-specific antibodies in vaginal wash were measured by ELISA (immune; n=19, immune/WT HSV-2; n=10, immune/HI HSV-2; n=9). Sample dilution for ELISA was 1:5. Data are mean ± SEM. Data are representative of two independent experiments (a-c) or are pooled from three independent experiments (d,e). Statistical significance was analyzed by two-tailed Mann-Whitney U test.

HHS Vulnerability Disclosure