Extended Data Fig. 10. Surface chemokine receptor on circulating memory B cells and validation of CXCR3 deficiency and its effect on the number of circulating memory B cells.

a, C57BL/6 mice were immunized ivag with TK⁻ HSV-2. Five weeks later, expression of various chemokine receptors on circulating IgD⁻IgG⁺ memory B cells in blood was analyzed by flow cytometry. b-c, C57BL/6 mice with (n=6) or without (n=5) immunization with TK⁻ HSV-2 five weeks prior were challenged with WT HSV-2 intravaginally. At the indicated days following challenge, CXCR3 expression on IgD⁻IgG⁺ memory B cells was analyzed by flow cytometry (b) and CXCL9 secretion in vaginal wash was measured by ELISA (c). d, Strategy for generating mixed BM chimeric mice. e-f, WT and CXCR3-B KO chimeric mice were immunized ivag with TK⁻ HSV-2 (n=10). e, Five weeks later, CXCR3 expression on IgD⁻IgG⁺ memory B cells, CD4 T cells, and CD8 T cells were analyzed by flow cytometry. f, Five weeks later, the number of circulating memory B cells, CD4 T cells and CD8 T cells was analyzed by flow cytometry. Data are mean ± SEM. Data are representative of four (a) and two (b,c) independent experiments or are pooled from two independent experiments (e,f). Statistical significance was analyzed by two-tailed Mann-Whitney U test.