Fig. 2. Rapid B cell recruitment into the FRT upon secondary challenge is a prerequisite for antibody secretion into vaginal lumen.

a-b, C57BL/6 mice were infected vaginally with TK⁻ HSV-2 five weeks prior. Mice were given drinking water containing 4 μg/ml of FTY720 for two weeks. a, One day after secondary challenge, the number of IgG⁺ MHC class II⁺ cells and CD11c^hi MHC class II^hi cells were analyzed by flow cytometry (n=3). b, After challenge, HSV-2-specific antibodies in vaginal wash were measured by ELISA (n=5). Sample dilution for ELISA was 1:7. Data are mean ± SEM. Data are representative of two (f) independent experiments, or are pooled from two (g) independent experiments. *, p<0.05 (two-tailed Mann-Whitney U test).