Fig. 3. CXCR3 is indispensable for recruitment of memory B cells into the vagina upon secondary challenge with HSV-2.

a, C57BL/6 mice were immunized ivag with TK⁻ HSV-2. Five weeks later, expression of various chemokine receptors on circulating IgD⁻IgG⁺ memory B cells or IgD⁺IgG⁻ naive B cells in blood was analyzed by flow cytometry. b, Eight hours after challenge in naïve or TK⁻ HSV-2 immunized mice, mRNA expression of various chemokines (relative to naïve mice) was measured in vaginal tissues by real-time qPCR (n=4 mice). c-d, Mixed BM chimeric mice were immunized intravaginally with TK⁻ HSV-2. Five weeks later, mice were challenged with WT HSV-2. c, Eighteen hours after challenge, the number of IgD⁺ naive B cells and IgG⁺ memory B cells in vaginal tissues was analyzed by flow cytometry (n=11). d, Eighteen hours after challenge, HSV-2-specific antibodies in vaginal wash were measured by ELISA (n=11). Sample dilution for ELISA was 1:10. Data are mean ± SEM. Data are representative of four (a) and two (b) independent experiments or are pooled from two independent experiments (c,d). ns, non-significant (two-tailed Mann-Whitney U test).