Extended Data Fig. 2. Vagina is devoid of virus-specific antibodies and tissue-resident B cells even after boosting local inflammation following immunization with HSV-2.

C57BL/6 mice were immunized intravaginally with TK⁻ HSV-2. On day 5 following immunization, CpG1826 was injected into the vagina to boost local inflammation of the vaginal mucosa. a, Five weeks later, the number of CD138⁺CD19⁺, CD138⁺CD19⁻, and CD138⁻CD19⁺ cells in vaginal tissues was analyzed by flow cytometry (naïve; n=4, immune; n=6, immune/CpG1826; n=5). b-c, HSV-2-specific antibodies in vaginal wash (b, naïve; n=4, immune; n=6, immune/CpG1826; n=5) and in serum (c, naïve; n=3, immune; n=3, immune/CpG1826; n=5) were measured by ELISA. Sample dilution for vaginal wash ELISA was 1:5. Data are mean ± SEM. Data are representative of two independent experiments. Statistical significance was analyzed by two-tailed Mann-Whitney U test.