Extended Data Fig. 3. B cells do not establish residency in the upper reproductive tract following immunization and challenge with HSV-2.

C57BL/6 mice were immunized intravaginally with TK⁻ HSV-2. Five weeks later, mice were challenged with ivag WT HSV-2. One day after, the number of CD138⁺CD19⁺, CD138⁺CD19⁻, and CD138⁻CD19⁺ cells in cervix and uterus was analyzed by flow cytometry (naïve; n=4, immune; n=6, immune/WT HSV-2; n=6). Data are mean ± SEM. Data are pooled from two independent experiments. Statistical significance was analyzed by two-tailed Mann-Whitney U test.