Extended Data Fig. 4. Surface marker profiles of recruited memory B cells in the vagina.

C57BL/6 mice immunized with TK⁻ HSV-2 5 wk prior were challenged with WT HSV-2. a, Eighteen hours later, CD80 and PD-L2 expression on both IgD⁺ and IgD⁻ B cells in vaginal tissues was analyzed by flow cytometry. b, The number of CD80⁺PD-L2⁺ memory B cells in the vagina on 18 hours after secondary challenge was analyzed by flow cytometry (naïve; n=2, immune; n=4). Data are mean± SEM. Data are representative of two independent experiments. Statistical significance was analyzed by two-tailed Mann-Whitney U test.