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Inhibition of DAG kinase or DAG lipase does not reduce the cell surface population of CCR2 in freshly isolated monocytes and THP‐1 cells. Three representative (n = 6) flow cytometry profiles of freshly isolated CD14+ monocytes (a) and THP‐1 cells (b) labelled with anti‐CCR2 antibodies or isotype control. Cells were treated with vehicle control, R59949 (30 μM), or RHC80267 (30 μM). Anti‐CCR2 cell surface immunoreactivity is indistinguishable between vehicle control and test groups
