Figure 5.

Effects of DAG kinase and DAG lipase inhibitors on CCL2‐evoked Ca²⁺ responses in THP‐1 cells in the absence of extracellular Ca²⁺. (a) Averaged (n = 8) intracellular Ca²⁺ responses evoked by CCL2 (50 ng·ml⁻¹) in the absence of extracellular Ca²⁺ and presence (open circles) and absence (closed circles) of R59949 (30 μM). (b) and (c) show the effect of R59949 on intracellular Ca²⁺ peak and AUC in the absence of extracellular Ca²⁺ (n = 8). (d) Averaged (n = 8) intracellular Ca²⁺ responses evoked by CCL2 (50 ng·ml⁻¹) in the absence of extracellular Ca²⁺ and presence (open circles) and absence (closed circles) of RHC80267 (30 μM). (e) and (f) show the effect of R59949 on intracellular Ca²⁺ peak and AUC in the absence of extracellular Ca²⁺ (n = 8). *P < .05 versus vehicle control for all