Figure 8.

Effect of DAG kinase α knock‐down of CCL2‐evoked Ca²⁺ responses in THP‐1 cells. (a) Averaged intracellular Ca²⁺ response evoked by CCL2 in THP‐1 cells transfected with scrambled siRNA (closed circles) or DAG kinase α targeted siRNA (open circles; n = 8). Bar charts showing peak (b) and AUC (c) intracellular Ca²⁺ responses evoked by CCL2 in scrambled, GAPDH knock‐down, or DAG kinase α knock‐down THP‐1 cells (n = 8). (d) qRT‐PCR analysis of GAPDH or DAG kinase α mRNA transcripts in scrambled and knock‐down THP‐1 cells (n = 8). F ratio is the Ca²⁺ response as measured by the Fura‐2 emission intensity ratio when excited at 340 and 380 nm. Responses evoked by 50‐ng·ml⁻¹ CCL2 for all experiments. *P < .05 versus scrambled control cells in all experiments. Data in each experiment are expressed as a percentage of the Ca²⁺ response evoked by ionomycin in control for cell number in knock‐down studies