Table 1.
Expression of DAG kinase, DAG lipase, and PKC mRNA transcripts in CD14+ monocytes and THP‐1 cells
| Gene | Name | CD14+ monocytes | THP‐1 cells |
|---|---|---|---|
| DGKA | DAG kinase α | Expressed | Expressed |
| Sense | CAACAGGAGGCAAAACTGGC | ||
| Anti‐sense | TGCCACCTAGAGATCCACGA | ||
| DGKB | DAG kinase β | Not expressed | Not expressed |
| Sense | TCTGTGTGCAATGGAGGCAT | ||
| Anti‐sense | CCACCCACAACAATCTGTCCA | ||
| DGKD | DAG kinase δ | Expressed | Expressed |
| Sense | TTATTGGTTGCACGCACAGC | ||
| Anti‐sense | CAGACCATCATCAAAGAGGGGA | ||
| DGKE | DAG kinase ε | Expressed | Expressed |
| Sense | AGAGGGTAAGTCTCGTCCCC | ||
| Anti‐sense | CTCTGGGAACAGGCAACGAT | ||
| DGKG | DAG kinase γ | Expressed | Expressed |
| Sense | GACTTCTCACCTGGCCTGTC | ||
| Anti‐sense | GTCCCTCGGTCTTCAAGCTC | ||
| DGKH | DAG kinase η | Expressed | Expressed |
| Sense | TATCCCCAGATCCAGGCTGT | ||
| Anti‐sense | CAGATGCAGCTATGCTCCCA | ||
| DGKI | DAG kinase ι | Not expressed | Not expressed |
| Sense | TCCAGTGTGACATGGGCATC | ||
| Anti‐sense | GGTACCTGAATCCACGGCAA | ||
| DGKK | DAG kinase κ | Expressed | Not expressed |
| Sense | CACTGGGCAATGCGATGATG | ||
| Anti‐sense | TAACCAGACAAGCCCACGAC | ||
| DGKQ | DAG kinase θ | Expressed | Expressed |
| Sense | AGGCTCCGAGAGTGACTGAT | ||
| Anti‐sense | GTGAACCCCAAGAGTGGAGG | ||
| DGKZ | DAG kinase ζ | Expressed | Expressed |
| Sense | GGCTCAGGTCGAAGACTTGT | ||
| Anti‐sense | CAGGATGCTGCAGAAGTCAGT | ||
| DAGLA | DAG lipase α | Expressed | Expressed |
| Sense | TGTCGGATGGCACAATGTCA | ||
| Anti‐sense | GAGCCCAGGATGGAGGTGG | ||
| DAGLB | DAG lipase β | Expressed | Expressed |
| Sense | GTTCGTACCACAAACCGTGC | ||
| Anti‐sense | CTCCCAGGAAGCTGATCTGGA | ||
| PRKCA | PKCα | Expressed | Expressed |
| Sense | CCTTTGCCACACACTTTGGG | ||
| Anti‐sense | TGACCGAGTGAAACTCACGG | ||
| PRKCB | PKCβ | Expressed | Expressed |
| Sense | TCTCTTGTCTCTAGCTTTTGGCT | ||
| Anti‐sense | CCCGGAAGAAAAGACGACCA | ||
| PRKCG | PKCγ | Not expressed | Not expressed |
| Sense | TCTAGAATGGGACAGGGGGT | ||
| Anti‐sense | CAGGACAGCCTCCCTTCGAT | ||
| PRKCD | PKCδ | Expressed | Expressed |
| Sense | TGTTGAGGCCCCAGACAAAG | ||
| Anti‐sense | TGGTGGTTGGTGCGTTGTAG | ||
| PRKCE | PKCε | Expressed | Expressed |
| Sense | GGTGCAGACTTGACACTGGT | ||
| Anti‐sense | GAACCCGGCGAGGAAATACA | ||
| PRKCH | PKCη | Not expressed | Expressed |
| Sense | GATCCACCCAACCCTCGAA | ||
| Anti‐sense | GCGGGGGTTCTGTGAACTTG | ||
| PRKCQ | PKCθ | Expressed | Expressed |
| Sense | TTAAGCAGCGAGCCTGTTGA | ||
| Anti‐sense | GAAACCTCAAGGCCGAATGC | ||
| PRKCI | PKCι | Expressed | Expressed |
| Sense | CCCTGGTGTTCATTGCCTCT | ||
| Anti‐sense | GGCCTTTGCAATGAGGTTCG | ||
| PRKCZ | PKCζ | Expressed | Expressed |
| Sense | TGGCTTAAGGTCCTCCGAGT | ||
| Anti‐sense | GAGTTCCGCGGAGTTGACC |
Note. Data are representative of five donors/independent experiments. Sense and anti‐sense primer sequences used for nonquantitative RT‐PCR are given beneath each gene assayed. Positive control PCR products were generated for all primers sets using human brain cDNA template.