Figure 5.

KB‐R7943 caused cell cycle arrest and apoptosis in glioblastoma cells. (a) U87 cells were incubated with 0.1% DMSO (vol/vol), BAPTA‐AM (10 μM), KB‐R7943 (25 μM), and BAPTA‐AM + KB‐R7943 for 18 hr and then underwent cell cycle analysis. (b) U87 cells underwent an apoptosis assay after incubation with 0.1% DMSO, 10‐μM BAPTA‐AM, 25‐μM KB‐R7943, and BAPTA‐AM + KB‐R7943 for 20 hr. (c) Cell fractions in G₀/G₁, S, and G₂/M phases of each group. KB‐R7943‐induced G₀/G₁ arrest was not rescued by BAPTA‐AM. n = 5 independent experiments; data were analysed by one‐way ANOVA. (d) Percentage of apoptotic cells in each group. KB‐R7943‐induced apoptosis was not attenuated by BAPTA‐AM. n = 5 independent experiments; data were analysed by one‐way ANOVA