Figure 1.

BAG6 knockdown disrupts translocation of mitochondria to the perinuclear region under depolarization. (A) Efficacy of BAG6 knockdown by siRNA in HeLa cells. HeLa cells were transfected with control or BAG6 siRNA. Three days after transfection, the expression of BAG6 protein was examined by western blotting. (B) Three days after transfection with siRNA, Flag‐Parkin‐transfected HeLa cells were treated with CCCP for 4 h and stained with anti‐TOMM20 (mitochondria; red), anti‐Flag (Parkin; green), and Hoechst (nucleus; blue). Scale bar represents 20 μm. (C) HeLa cells were transfected with Flag‐Parkin together with control or BAG6 siRNA #1, followed by treatment of 80 μg·mL⁻¹ antimycin A for 4 h. Cells were stained with anti‐TOMM20 (mitochondria; red), anti‐Flag (Parkin; green), and Hoechst (nucleus; blue). Scale bar represents 20 μm. (D) Immunocytochemistry with TOMM20 of BAG6 knockdown and control HeLa cells without ectopic expression of Parkin. Cells were treated with 20 μm CCCP for 4 h or DMSO (negative control). Scale bar represents 20 μm.