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Expression of Smurf1 in cells transfected with miR‐125a. (A) The Smurf1 protein expression and quantification in CT26 cells transfected with miR‐125a or control was determined by western blotting. ** P < 0.01. (B) Quantification of Smurf1 mRNA levels was determined by reverse transcription‐quantitative polymerase chain reaction. Data are presented as mean ± SD. Student's t‐test. ** P < 0.01.
