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. 2019 Jun 2;9(7):1249–1258. doi: 10.1002/2211-5463.12655

Figure 2.

Figure 2

X‐box binding protein 1 suppresses HG‐induced oxidative stress in MCs. After 24 h of transfection with an XBP1 plasmid (XBP1) or empty vector (vector), MCs were treated with HG for 48 h. (A) Intracellular generation of ROS by transforming H2DCF‐DA to DCF via an oxidative reaction. Scale bars: 100 μm. (B) Quantification data for the ROS level of each group. (C) SOD1 activity detection. (D) CAT activity detection. After 24 h of transfection with XBP1‐specific siRNA (si‐XBP1) or negative control (si‐Con), MCs were treated with HG for 48 h. (E) Quantification data for the ROS level of each group. (F) SOD1 activity detection. Data are represented as the mean ± SEM. The experiment was conducted in triplicate. **P < 0.01 vs. the control group; # P < 0.05 vs. the vector + HG group or the si‐Con + HG group. One‐way ANOVA with Tukey's post hoc test.