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. 2019 Jun 4;9(7):1223–1231. doi: 10.1002/2211-5463.12651

Figure 3.

Figure 3

Relationship between TCTN2 and miR‐216b. RNA immunoprecipitation (RIP) and RNA pull‐down assay were performed to explore the interaction between TCTN2 and miR‐216b in SY‐SH‐5Y cells. (A) Bioinformatics analysis (DIANA) predicted the binding sites between TCTN2 and miR‐216b. (B) RIP assay was performed to determine the binding condition between TCTN2 and miR‐216b, and immunoprecipitation–western blotting was performed to determine whether TCTN2 and miR‐216b are present in the AGO2 protein complex. (C) RNA pull‐down assay was conducted to examine the interaction between TCTN2 and miR‐216b. *P < 0.05 compared with Input or negative control (NC). The error bars indicate SD. The experiments were repeated three times. Comparisons between the two groups were analyzed with Student's t test.