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. 2019 Jun 4;9(7):1223–1231. doi: 10.1002/2211-5463.12651

Figure 4.

Figure 4

TCTN2 regulated autophagy and apoptosis through miR‐216b–Beclin‐1 pathway. SY‐SH‐5Y cells were assigned to six groups: control, OGD, OGD+pcDNA, OGD+pcDNA‐TCTN2, OGD+pcDNA‐TCTN2+pre‐NC, and OGD+pcDNA‐TCTN2+miR‐216b mimic. (A) Forty‐eight hours after cell transfection, the expression of miR‐216b and Beclin‐1 protein was evaluated with qRT‐PCR and western blot, separately. (B) The expression of P62, LC3I, and LC3II protein in SY‐SH‐5Y cells was assessed with western blot. (C) SY‐SH‐5Y cell apoptosis was estimated with flow cytometric analysis. *P < 0.05 compared with control; # P < 0.05 compared with OGD+pcDNA; & P < 0.05 compared with OGD+pcDNA‐TCTN2+pre‐NC. The error bars indicate SD. The experiments were repeated three times. Comparisons between the two groups were analyzed with Student's t test.